CK 8/18
Use
Cytokeratins (CK) 8 and 18 are major components of the intermediate filaments in thyroid, liver, and most simple epithelia. CK8/18 is used as a marker to aid in the identification of adenocarcinomas and squamous cell carcinomas, Paget cells, metastatic gastric, and colorectal carcinomas.
Special Instructions
Fresh tissue specimens should be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol for long-term storage. Unstained slides must be on positively charged glass to prevent background staining and tissue loss. Testing should occur within three months of placement in ethanol.
Limitations
Specification of the fixation time is critical for accurate results. The test is not suitable for tissue not fixed in 10% neutral buffered formalin or B plus.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin-fixed Paraffin Embedded Tissue (FFPE blocks)
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Store at ambient temperature with a refrigerated gel pack. Do not freeze.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | Indefinite |