Colloidal Iron Stain
Use
Colloidal iron special stain is used to detect acid mucopolysaccharides and sialomucins in tissue samples. It is particularly useful for identifying mucins in gastrointestinal tract tissues and helps distinguish chromophobe renal cell carcinoma from other renal tumors. Additionally, it is utilized to detect Cryptococcus neoformans, a fungal infection that primarily affects immunocompromised individuals.
Special Instructions
Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% neutral buffered formalin or B plus. All other fixatives will result in specimen rejection. Fresh tissue specimens should not exceed 72 hours of storage at ambient temperature prior to fixation. Improperly prepared slides may result in background staining or tissue loss.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed or Unstained slides
Collection Instructions
Fresh tissue fixative should be at a 20:1 fixative to tissue ratio, 10% NBF for 6-72 hours. Unstained slides should be positively charged to prevent background staining.
Storage Instructions
FFPE tissues can be stored indefinitely at 15°C to 25°C. Fresh tissue is stable for ≤ 72 hours and should be processed promptly.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite (FFPE); ≤ 72 Hours (Fresh Tissue) |