Colloidal Iron Stain, technical only
Use
Colloidal iron special stain is used to detect acid mucopolysaccharides and sialomucins in tissue samples. This stain is particularly useful for identifying mucins in gastrointestinal tract tissues and has been employed to distinguish chromophobe renal cell carcinoma from other renal tumors. Additionally, colloidal iron staining is utilized to detect Cryptococcus neoformans, a fungal infection that primarily affects immunocompromised individuals.
Special Instructions
Technical component only. Pathologist read not included. Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% neutral buffered formalin (NBF) or B plus. All other fixatives will be rejected. Fresh Tissue should be stored and shipped ambient; cannot be frozen.
Methodology
Other
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Storage Instructions
Store fresh tissue specimens at 15°C to 25°C. Transfer to 70% alcohol for transport if needed.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |