Congo Red Stain
Use
Congo red special stain is used to detect amyloid deposits in tissue sections. Congo red binds to amyloid fibrils, and under polarized light, these deposits exhibit characteristic apple-green birefringence, aiding in the diagnosis of amyloidosis. This staining is essential for identifying amyloid-related diseases, as it highlights the presence of amyloid deposits that may not be visible with routine histological stains.
Special Instructions
Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. Fresh Tissue must be stored ambient with a refrigerated (not frozen) gel pack in the shipment. Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides should be protected from extreme temperatures during shipping.
Methodology
Anatomic Pathology
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |