Cyclin D1/PRAD1, technical only
Use
Cyclin D1 is a cell cycle regulator that controls the transition from G1 to S phase in normal tissues. A mutation in this protein leads to increased cell proliferation, disrupting downstream pathways, and resulting in tumorigenesis. Immunohistochemistry detection is valuable in diagnosing and prognosing various tumors, including mantle cell lymphoma.
Special Instructions
Not provided.
Limitations
Specimens must be fixed in 10% NBF or B plus; other fixatives are rejected. Fresh tissues must be tested within 3 months after placing in ethanol for storage. Positively charged glass slides are required to prevent background staining and tissue loss. Unstained slides should not be baked.
Methodology
Immunoassay (IHC)
Biomarkers
Cyclin D1
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours PREFERRED).
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |