Epstein Barr Virus Early RNA, technical only
Use
EBER ISH is an in situ hybridization assay that stains latent Epstein-Barr virus (EBV) in tissues. It is used to detect the presence of EBV within tissue samples, which is important for diagnosing conditions associated with EBV infections. This assay is for technical component only, meaning that it involves the actual testing procedure but does not include a pathologist's interpretation or reading of the test results.
Special Instructions
Fresh tissues should be fixed in formalin for 24-72 hours for sites that collect samples. These can then be transferred to 70% ethanol and stored at 2-8°C if needed for longer-term storage. Samples should be tested within 3 months of being placed in ethanol. Unstained slides should be positively charged to prevent background staining and prevent tissue loss. Slides should be left unbaked for optimal results.
Limitations
The test does not include a pathologist read or interpretation, limiting its use to technical analysis only. Specimens must be fixed in 10% neutral buffered formalin or B plus; other fixatives will result in specimen rejection. Testing should occur within 3 months after transferring to ethanol for best results. The test is technical-only and does not include diagnostic interpretation results.
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C; ambient shipping with refrigerated (not frozen) gel packs optional.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus; all other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |