ETV6::RUNX1; t(12;21)
Use
This FISH test is used to detect the gene fusion ETV6::RUNX1 (TEL::AML1), associated with the chromosomal translocation t(12;21). It is the most common genetic abnormality in childhood B-acute lymphoblastic leukemia (ALL), present in 20-30% of cases. The test is valuable in identifying this subtle abnormality which is not easily detected with conventional cytogenetic banding techniques.
Special Instructions
All attempts will be made to process and report samples received more than 72 hours post-collection. Bone marrow is the preferred sample type for most hematologic malignancies. Shipping should use a refrigerated gel pack, and specimens should not be frozen.
Limitations
Turnaround times may vary based on clinical use and biopharma protocol requirements. The test's sensitivity is dependent on specimen quality, and results may be inconclusive if specimens are clotted or exposed to extreme temperatures. Specimens with insufficient cell numbers or toxic anticoagulants will be rejected.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
Not provided
Container
EDTA preferred (NaHep accepted)
Storage Instructions
2°C to 25°C
Causes for Rejection
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |