GCDFP-15, BRST-2 protein
Use
Gross cystic disease fluid protein 15 (GCDFP-15) is one of four major component proteins found in the cystic fluid of patients with fibrocystic changes of the breast. GCDFP-15 is a marker of apocrine glandular differentiation in benign and malignant mammary epithelium.
Special Instructions
Fresh tissues should be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides should be positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
The test's turnaround times are subject to change based on biopharma protocol requirements. Final turnaround times will be specified in the biopharma study contract. The use of fixatives other than 10% neutral buffered formalin or B plus can lead to specimen rejection.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 2-8°C for longer term storage if needed.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Refrigerated | Indefinite |
| Frozen | Indefinite |