Gordon & Sweets Special Stain (Reticulum)
Use
Gordon and Sweet's reticulin stain is a silver impregnation technique used to visualize reticular fibers, which are a type of connective tissue fiber. This method results in black-stained fibers against a pale background. It is particularly useful in liver histopathology for assessing the architecture of the liver and identifying conditions such as cirrhosis.
Special Instructions
Fresh Tissue specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol for long-term storage if needed. Unstained slides require positively charged glass slides to prevent background staining and tissue loss. They should be left unbaked.
Limitations
Stated turnaround times are for clinical use and are subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Methodology
Other
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |