Gordon & Sweets Special Stain (Reticulum), technical only
Use
Gordon and Sweet's reticulin stain is a silver impregnation technique used to visualize reticular fibers, particularly in liver histopathology. It is useful for assessing the architecture of the liver and identifying conditions such as cirrhosis. This test is technical only and does not include a pathologist read.
Special Instructions
Specimens should be fixed in 10% neutral buffered formalin at a 20:1 fixative to tissue ratio for 6-72 hours, with 48-72 hours preferred. Fresh tissues are to be stored at 15°C to 25°C. Slides should be positively charged to prevent background staining and tissue loss.
Limitations
The test is technical only, which means it does not include a pathologist read. It is for research purposes and clinical trials only, not for routine clinical diagnostics. The preferred fixation method is 10% neutral buffered formalin. All other fixatives will lead to specimen rejection.
Methodology
Other
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |