Granzyme B
Use
Granzyme B is a neutral serine protease stored in specialized lytic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. When released by CTL and NK cells, Granzyme B induces DNA fragmentation and apoptosis in target cells.
Special Instructions
Fresh tissues should be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Unstained slides should be positively charged to prevent background staining and tissue loss.
Limitations
Specimens must be fixed in 10% neutral buffered formalin or B plus. All other fixatives will be rejected. Specimens should be tested within 3 months after being placed in ethanol. Final turnaround times will be specified in the biopharma study contract and are subject to change based on protocol requirements.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Container
Unstained slides, Formalin Fixed
Collection Instructions
Fresh tissues should be fixed at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C. May be transferred to 70% alcohol for transportation.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours for fresh tissues; Indefinite for FFPE blocks and slides |