Hematoxylin & Eosin Stain
Use
Hematoxylin and eosin (H&E) is one of the most commonly used special stains in histopathology for routine examination of tissue sections. Hematoxylin stains cell nuclei a blue to purple color, while eosin stains the cytoplasm and extracellular matrix in shades of pink, allowing pathologists to examine tissue morphology and structure. This stain is crucial in identifying various pathological conditions, including tumors, infections, and inflammatory diseases, making it a fundamental tool in diagnostic pathology.
Special Instructions
Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol. Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Turn-around-times (TATs) are subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract. Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Methodology
Other
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
15°C to 25°C
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |