HEP Par 1, technical only
Use
Hepatocyte paraffin 1 (HepPar-1) is a monoclonal antibody that specifically detects a cytoplasmic protein expressed in hepatocytes and some other liver-related cells. In pathology, HepPar-1 is used to differentiate liver-derived tumors from other malignancies. This marker is especially valuable in cases where the primary tumor's origin is uncertain, helping to confirm a hepatic origin. Technical component only. Pathologist read not included.
Special Instructions
Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
This test is technical only; a pathologist's interpretation is not included. Fresh tissues must be fixed in 10% neutral buffered formalin or a similar preservative. Non-compliant specimens or those using different fixatives will be rejected.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |