Herpes Simplex Virus Type 2
Use
Qualitative real-time PCR assay for the detection of Herpes simplex virus type 2 (HSV-2). HSV-2 primarily causes genital herpes, characterized by painful sores and a risk for transmission during sexual contact. Clinically, HSV-2 is important due to its association with recurrent genital outbreaks, increased risk of HIV transmission, and complications during pregnancy, such as neonatal herpes.
Special Instructions
Fresh tissue: submit in transport medium such as RPMI, Hanks, or equivalent. Frozen tissue: freeze within 4 hours of collection.
Limitations
Detection via PCR is highly specific and sensitive compared to other methods. Non-detection does not exclude infection; false negatives may occur if viral DNA is not present at the time of sample collection or if the specimen is improperly handled.
Methodology
PCR-based (RT-PCR)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Cervical Swab
Volume
2.0 mL
Minimum Volume
1.0 mL
Container
ThinPrep® PAP solution or SurePath™ Test Pack solution
Collection Instructions
Use either ThinPrep® or SurePath™ solutions. Ensure the swab is fully immersed.
Storage Instructions
Store at ambient temperature (18-25°C).
Causes for Rejection
Specimens beyond stability; recommended temperatures exceeded; improper transport media; improper anticoagulant
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 96 hours |