HMB 45
Use
The human melanoma black (HMB) antibody reacts with neuraminidase-sensitive oligosaccharide side chains of glycoconjugates present in immature melanosomes, cutaneous melanocytes, prenatal/infantile retinal pigment epithelium (RPE), and melanoma cells. Studies support the routine use of HMB 45 as a sensitive and specific melanocytic marker.
Special Instructions
- Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. - Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. Fixatives other than specified will be rejected. Stated turnaround times are for clinical use only and may change based on biopharma protocol requirements.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |