HMB45 Red
Use
The human melanoma black (HMB) antibody reacts with neuraminidase-sensitive oligosaccharide side chains of glycoconjugates present in immature melanosomes, cutaneous melanocytes, prenatal/infantile retinal pigment epithelium (RPE), and melanoma cells. Studies support the routine use of HMB45 as a sensitive and specific melanocytic marker. Detection with Red Chromogen.
Special Instructions
Specimens may be transferred to 70% alcohol for transportation purposes after fixation. Positively charged glass slides are required to prevent background staining and tissue loss. Slides should remain unbaked. Samples must be tested within 3 months after placing in ethanol.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. The stated turnaround times (TATs) are for clinical use and subject to change based on specific biopharma protocols.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Store at 15°C to 25°C
Causes for Rejection
Specimens not fixed in 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |