IGH::BCL2; t(14;18)
Use
The IGH::BCL2 FISH test is designed to detect the fusion of the IGH and BCL2 genes, associated with the t(14;18) translocation. This genetic abnormality is frequently seen in B-cell malignancies such as follicular lymphoma and diffuse large B-cell lymphoma (DLBCL), making it a valuable tool in diagnosing and evaluating these types of lymphomas. It is often ordered as part of the F BURKITT Panel test for comprehensive evaluation of hematologic specimens.
Special Instructions
Efforts will be made to process and report samples received over 72 hours post-collection, although bone marrow is typically preferred in hematologic malignancy cases.
Limitations
The test depends on the availability of a sufficient number of viable cells for analysis. Specimens subjected to extreme temperatures, improper anticoagulants, or those that are clotted, may not yield reliable results. The analytical sensitivity and specificity of the FISH test may vary based on specimen quality and preparation technique.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
Not provided
Container
EDTA preferred (NaHep accepted)
Causes for Rejection
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 72 hours |