Inhibin
Use
Inhibin is a dimeric glycoprotein consisting of alpha and beta subunits that is mainly produced in the gonads. It regulates follicle stimulating hormone (FSH) secretion through negative feedback mechanisms with paracrine and autocrine actions on gonads and adrenal glands. Inhibin is highly expressed in sex cord stromal tumors, including ovarian granulosa cell tumors (GCT) and Sertoli-Leydig cell tumors, where it serves as a key marker for diagnosis and prognosis.
Special Instructions
Specimens may be fixed in formalin for 24-72 hours by collection sites, and then transferred to 70% ethanol, stored at 2-8°C for longer term storage if needed. Unstained slides require the use of positively charged glass slides to prevent background staining and tissue loss. It is important for samples to be tested within 3 months of being placed in ethanol.
Limitations
Specimens not fixed in 10% NBF or B plus will be rejected. The test's effectiveness depends on proper specimen fixation and slide preparation. Fresh tissues must be fixed promptly and properly to ensure test accuracy. Tissue samples stored for too long or improperly may result in inaccurate or invalid results.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
8 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Refrigerated | Indefinite |
| Frozen | Indefinite |