Inhibin, technical only
Use
Inhibin is a dimeric glycoprotein primarily produced in the gonads, regulating follicle-stimulating hormone secretion through negative feedback. It is highly expressed in sex cord stromal tumors, such as ovarian granulosa cell tumors and Sertoli-Leydig cell tumors. The test is used to measure inhibin levels, assisting in the diagnosis and monitoring of these tumors.
Special Instructions
This test provides the technical component only and does not include a pathologist read. Fresh tissues require a formalin fixation ratio of 20:1 formalin to tissue for 6-72 hours, ideally 48-72 hours in 10% neutral buffered formalin.
Limitations
The test is limited to the technical component using immunohistochemistry. Fixatives other than 10% neutral buffered formalin or B plus will result in specimen rejection. The test is specific to detecting inhibin in pre-defined contexts and may not account for other conditions where inhibin is altered.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Specimens should be fixed in 10% neutral buffered formalin with a ratio of 20:1 fixative to tissue for 6-72 hours, with 48-72 hours preferred. Alternatively, specimens can be transferred to 70% alcohol after initial formalin fixation for transportation.
Storage Instructions
Store specimens at 15°C to 25°C. Fresh tissues should be processed within 72 hours. FFPE blocks and unstained slides have indefinite stability.
Causes for Rejection
Specimens using fixatives other than 10% neutral buffered formalin or B plus are rejected. Also, improper handling or preparation may result in rejection.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours for Fresh Tissue; Indefinite for FFPE/unstained slides |