Kappa/Lambda Dual ISH
Use
Kappa and lambda light chains are components of immunoglobulins produced by B cells. The ratio of these light chains can provide insights into various hematological conditions. An atypical ratio of kappa to lambda light chains can indicate the presence of plasma cell neoplasms.
Special Instructions
Fresh Tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples must be tested within 3 months after placing in ethanol. Unstained slides require positively charged glass slides to prevent background staining and tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. The test turnaround time is subject to change based on biopharma protocol requirements and the final TAT will be specified in the biopharma study contract.
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fresh Tissues: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Formalin-fixed Paraffin Embedded Tissue (FFPE blocks) and unstained slides: Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Storage Instructions
Stored at 15°C to 25°C
Causes for Rejection
All other fixatives except 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |