Ki67, technical only
Use
Ki-67 is a nuclear protein associated with cellular proliferation. In pathology, Ki-67 is widely used to assess the proliferation rate of tumor cells, which can be an important indicator of tumor aggressiveness and prognosis. High Ki-67 expression is often correlated with more aggressive cancers, and its evaluation can guide treatment decisions, particularly in assessing the need for chemotherapy.
Special Instructions
Technical component only. Pathologist read not included. Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. Fresh Tissues should be fixed for 6-72 hours (48-72 hours preferred). Unstained slides should be provided as 2 slides (3-5 μM) per stain (min 1 slide per stain), ensuring they are left unbaked. Storage conditions require ambient temperature and use of a gel pack during shipping to protect from extreme temperatures.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C, ambient
Causes for Rejection
All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |