Lambda Immunoglobulin Light Chain, technical only
Use
Lambda ISH is an in situ hybridization assay that targets the lambda immunoglobulin light chain on plasma cells or lymphocytes. This assay is used to establish clonality and detect plasma cell neoplasms. As a technical component only, the test does not include a pathologist read, making it crucial for laboratories to pair this assay with appropriate interpretative services.
Special Instructions
Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol for longer-term storage if needed. Unstained slides require positively charged glass slides to prevent background staining and tissue loss. Slides should be left unbaked.
Limitations
The test is a technical component only, which means it does not include pathologist interpretations. Interpretation of results must be done separately by a qualified pathologist. Pre-analytical variables in specimen handling, fixation, and storage can affect the quality and readability of in situ hybridization results. Fixatives other than specified 10% NBF or B plus will lead to specimen rejection. Stability of fresh tissues is limited to 72 hours, after which quality may be compromised.
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Storage Instructions
Store at 15°C to 25°C
Causes for Rejection
Specimens fixed in any fixative other than 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |