Melanin Stain, technical only
Use
Melanin special stain is used to detect melanin pigment in tissue sections. This silver stain reacts with melanin to produce a black coloration, facilitating the identification of melanocytic lesions, such as melanoma, and assessing pigmentation in various tissues. Additionally, this stain can also detect other reducing substances, including argentaffin and chromaffin pigments, providing valuable diagnostic information.
Special Instructions
Technical component only. Pathologist read not included. Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol.
Limitations
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract. Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. Positively charged glass slides are required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Methodology
Other (Anatomic Pathology)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Frozen | ≤ 72 Hours |