Nectin-4
Use
Nectin-4 is a cell adhesion molecule predominantly expressed during embryonic development and re-expressed in various cancers, including bladder, breast, and lung cancers. Its overexpression is associated with tumor proliferation, angiogenesis, and poor prognosis. Clinically, Nectin-4 serves as a diagnostic and prognostic biomarker and is a target for antibody drug conjugates.
Special Instructions
Fresh tissues should be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides require positively charged glass to prevent background staining and tissue loss. Slides should be left unbaked.
Limitations
Stated turn-around-times are for clinical use only and may change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |