Neurofilament
Use
Neurofilament is a major cytoskeletal element consisting of three distinct polypeptides (neurofilament triplet) found in nerve axons and dendrites. Metabolism appears to be disrupted in Alzheimer's disease, as evidenced by the presence of neurofilament epitopes in neurofibrillary tangles and a severe reduction in gene expression for the light neurofilament subunit of the neurofilament triplet in the brains of Alzheimer's patients.
Special Instructions
Fresh Tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Samples must be tested within 3 months after placing in ethanol. Unstained slides must be positively charged glass slides to prevent background staining and tissue loss. Slides should remain unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. Any other fixatives will result in rejection. Fresh tissues have a stability of ≤ 72 hours, while formalin-fixed tissues and unstained slides have indefinite stability.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
8 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Specimens may be transferred to 70% alcohol for transportation purposes after fixation.
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Refrigerated | 2-8°C for longer term storage. |
| Frozen | Samples must be tested 3 months after placing in ethanol. |