Neurofilament, technical only
Use
Neurofilament is a major cytoskeletal element consisting of three distinct polypeptides found in nerve axons and dendrites. Its metabolism is disturbed in Alzheimer's disease, as indicated by the presence of neurofilament epitopes in neurofibrillary tangles and by a severe reduction of gene expression for the light neurofilament subunit in Alzheimer's patients. This test is technical only and does not include a pathologist read.
Special Instructions
Specimens may be fixed in formalin for 24-72 hours by collection sites and then transferred to 70% ethanol for long-term storage at 2-8°C. Samples must be tested within 3 months after placing in ethanol. Unstained slides should be on positively charged glass slides to prevent background staining and tissue loss.
Limitations
The test is limited to technical component analysis only and does not include a pathologist's interpretation. Specimens fixed in non-standard fixatives will be rejected. Storage conditions and fixation time could affect the stability and integrity of the specimens, influencing the accuracy of the test results. Fresh tissues have a stability of 72 hours, while FFPE blocks and unstained slides are stable indefinitely when stored properly.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Container
Fixative at 20:1 fixative to tissue ratio, 10% NBF
Collection Instructions
Fix in 10% neutral buffered formalin for 6-72 hours, 48-72 hours preferred
Storage Instructions
Store at 15°C to 25°C. Transfer to 70% alcohol for transport if needed.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus are rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤72 Hours |
| Refrigerated | Indefinite |
| Frozen | Indefinite |