Neuron Specific Enolase, technical only
Use
Neuron specific enolase is a cytoplasmic enzyme expressed by neuroendocrine cells and tumors, including poorly differentiated neuroendocrine tumors. This test evaluates the technical component only and does not include a pathologist read.
Special Instructions
Stated turnaround times (TATs) are for clinical use only and are subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract. Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Unstained slides require positively charged glass slides to prevent background staining and tissue loss.
Limitations
Specimens must be fixed in 10% neutral buffered formalin (NBF) or B plus. All other fixatives will result in specimen rejection. The testing is limited to the technical component and excludes pathologist interpretation.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours, preferably 48-72 hours.
Storage Instructions
Store at 15°C to 25°C. Fresh tissues should be stored at 2-8°C after transfer to 70% ethanol if needed.
Causes for Rejection
Specimens fixed in fixatives other than 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |