OCT-2, technical only
Use
OCT-2 is a B-cell restricted transcription factor of the POU homeo-domain family that binds the Ig gene to regulate B-cell-specific genes. It is found in several B-cell neoplasms including myeloma, differentiate nodular lymphocyte predominant Hodgkin lymphoma (NLPHL), and classical Hodgkin lymphoma (CHL). The test is technical component only, meaning that it does not include the pathologist read, which may be critical for full diagnostic purposes.
Special Instructions
Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Samples must be tested within 3 months after placing in ethanol. Positively charged glass slides are required to prevent background staining and tissue loss. Slides should be left unbaked.
Limitations
The test is technical only and does not include a pathologist read, which may limit its clinical use without proper clinical correlation. Additionally, specimens not fixed in 10% neutral buffered formalin (NBF) or B plus will be rejected, limiting the test's applicability to properly prepared specimens.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Storage Instructions
Specimens may be transferred to 70% alcohol for transportation after fixation.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |