p16
Use
p16 is a tumor suppressor protein encoded by the CDKN2A gene. Positive staining or overexpression of this protein correlates with oncogenic HPV infection and HPV-independent mechanisms found in head and neck cancers as well as anogenital lesions. This test is used in the identification of these cancers and can aid in the evaluation of tumor characteristics in a clinical setting.
Special Instructions
Fresh tissues should be fixed at a ratio of 20:1 fixative to tissue using 10% neutral buffered formalin for 6-72 hours, with 48-72 hours preferred. Specimens may be transferred to 70% ethanol for transportation after fixation. Unstained slides should be on positively charged glass slides to prevent background staining and tissue loss, remaining unbaked before use.
Limitations
The test's effectiveness in identifying the presence of p16 as a marker for HPV-related cancers may be limited if tissue samples are not correctly prepared (e.g., wrong fixatives). Samples fixed in any medium other than 10% neutral buffered formalin will be rejected. While FFPE samples offer indefinite stability, fresh tissues must be used within 72 hours to maintain integrity. Accuracy of results is contingent on adherence to these specimen handling and fixation guidelines.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |