P53, technical only
Use
p53 is a critical tumor suppressor protein involved in cell cycle control by halting abnormal growth in normal cells. Abnormal activity results from mutations in the TP53 gene found in a variety of tumor types. Immunostaining closely correlates with the presence of a mutation. Technical component only. Pathologist read not included.
Special Instructions
Not provided.
Limitations
This test only detects abnormal p53 activity related to mutations in the TP53 gene. It does not include a pathologist interpretation. Only technical component is provided, which may require follow-up with comprehensive pathology read.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. For fresh tissues, specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |