P53, technical only
Use
p53 is a critical tumor suppressor protein involved in cell cycle control by halting abnormal growth in normal cells. Abnormal activity results from mutations in the TP53 gene found in a variety of tumor types. Immunostaining closely correlates with the presence of a mutation. Technical component only. Pathologist read not included.
Special Instructions
Specimens may require specific fixation and storage procedures to ensure validity of results. Use ambient conditions for shipping and handle specimens with care to avoid rejection due to improper fixatives.
Limitations
This test only detects abnormal p53 activity related to mutations in the TP53 gene. It does not include a pathologist interpretation. Only technical component is provided, which may require follow-up with comprehensive pathology read.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. For fresh tissues, specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |