Pan-TRK
Use
The Pan-TRK test is designed for the detection of TRK proteins A, B, and C. Tropomyosin receptor kinase (TRK) proteins are crucial for neural development and function, playing roles in the survival, growth, and death of cells. The test uses immunohistochemistry to identify the presence of these TRK proteins, particularly focusing on the C-terminal region. Understanding TRK expression is significant in diagnosing and determining the progression of cancer and other diseases associated with neurotrophic pathways.
Special Instructions
Fresh tissue specimens may be fixed in formalin for 24-72 hours before being transferred to 70% ethanol for long-term storage. Unstained slides should be positively charged to avoid background staining and tissue loss, and should not be baked. This ensures the integrity of tissue samples for accurate testing.
Limitations
Specimens must be fixed in 10% neutral buffered formalin or B plus. Other fixation methods will lead to rejection of samples. The test is specifically designed to detect the TRK proteins A, B, and C and does not provide information on TRK protein mutations or variants. Results may be influenced by the quality of tissue preservation and preparation.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Ensure specimen is fixed in 10% neutral buffered formalin for 6-72 hours; 48-72 hours is preferred.
Storage Instructions
Store at 15°C to 25°C. Ambient shipping conditions.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | ≤ 72 Hours |