Paroxysmal Nocturnal Hemoglobinuria (PNH) by FLAER (High Sensitivity)
Use
Paroxysmal Nocturnal Hemoglobinuria (PNH) is a high sensitivity flow cytometry test that uses fluorescence labeled proaerolysin (FLAER) to diagnose PNH by identifying abnormal red blood cell populations that lack certain surface proteins, such as CD55 and CD59, due to a genetic mutation. This test may also be used to diagnose glucose phosphate isomerase (GPI) deficiency. It is relevant for patients with Coombs negative hemolytic anemia, myelodysplastic syndromes with unilineage dysplasia, unexplained hemoglobinuria, aplastic anemia, cytopenia, or thrombosis.
Special Instructions
Not provided.
Limitations
The test requires a minimum blood volume of 0.5 mL with 107 cells. Specimen stability is limited to 48 hours at 4°C. It cannot use specimens stored at incorrect temperatures, non-viable specimens, or specimens with inappropriate anticoagulants. Hemolysis and clotting result in rejection of the specimen.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
CD55
ProteinCD59
Protein
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.5 mL with 107 cells
Container
NaHep preferred (EDTA accepted)
Storage Instructions
2°C to 8°C
Causes for Rejection
Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 48 hours at 4°C |