PAX-8, technical only
Use
PAX-8 is a transcription factor involved in thyroid follicular cell development and expression of thyroid-specific genes. It is a useful marker in a panel of antibodies for detecting thyroid, ovarian, endometrioid, renal epithelial, and clear cell carcinomas. This test offers the technical component only, with the pathologist read not included.
Special Instructions
Fresh tissues should be fixed at a 20:1 fixative to tissue ratio in 10% neutral buffered formalin for 6-72 hours, with 48-72 hours preferred. Use positively charged glass slides for unstained slides to prevent background staining and tissue loss. Slides should be left unbaked. Samples can be stored in 70% ethanol at 2-8°C after fixation for long-term storage.
Limitations
The test is technical only and does not include a pathologist read. Specimens must be fixed in 10% neutral buffered formalin or B plus; other fixatives will lead to rejection. Stated turnaround times may vary based on biopharma protocol requirements and will be specified in the biopharma study contract.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store between 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 hours |