PBMC Isolation
Use
Peripheral blood mononuclear cell (PBMC) processing involves the isolation of mononuclear cells, including lymphocytes and monocytes, from whole blood through density gradient centrifugation. This process is used to assess immune responses, biomarker identification, and drug efficacy.
Special Instructions
This test is available for use in clinical trials or research purposes only. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Limitations
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Methodology
Cell-based / Cytometry
Biomarkers
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
Not provided
Minimum Volume
Not provided
Container
NaHep, EDTA, or LiHep (dependent on protocol)
Storage Instructions
Store at room temperature prior to PBMC isolation. Isolated PBMCs stored in liquid nitrogen long term.
Causes for Rejection
Clotted specimens; whole blood collected in the wrong anti-coagulant; hemolyzed or clotted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Best results achieved if processing begins within 4 hours of blood collection. |