PDGFR
Use
Platelet-derived growth factor receptor (PDGFR) is a cell surface receptor involved in cell growth, development, and wound healing. In pathology, PDGFR is used to identify tumors such as gastrointestinal stromal tumors, where mutations in PDGFR can drive tumorigenesis. The detection of PDGFR expression and mutations can inform the use of targeted therapies, such as tyrosine kinase inhibitors.
Special Instructions
Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides should be positively charged to prevent background staining and tissue loss. Slides should remain unbaked.
Limitations
Specimens not fixed in 10% NBF or B plus will be rejected. Turnaround times are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
8 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Ambient; specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |