PDGFR, technical only
Use
Platelet-derived growth factor receptor (PDGFR) is a critical cell surface receptor that plays a significant role in cell growth, development, and wound healing. In the field of pathology, PDGFR is utilized to identify specific tumors, such as gastrointestinal stromal tumors, where mutations in PDGFR contribute to tumorigenesis. Detecting PDGFR expression and mutations is essential as it can guide targeted therapy options, including the use of tyrosine kinase inhibitors.
Special Instructions
This test is a technical component only and does not include a pathologist's interpretation. It requires special handling of specimens, ensuring they are fixed correctly and stored or transported under ambient conditions with appropriate precautions.
Limitations
This test does not include a pathologist's interpretation and is strictly technical. Incorrect specimen handling, such as using fixatives other than 10% NBF or B plus, will lead to specimen rejection. The test outcomes must be evaluated in conjunction with a complete clinical picture and may not be applicable for all tumor types or mutations.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours, with 48-72 hours preferred. Transfer to 70% ethanol after fixation if needed.
Storage Instructions
Specimens should be stored at ambient temperature (15°C to 25°C). For long-term storage, especially for unstained slides, use positively charged glass slides to prevent staining issues or tissue loss.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus, use of other fixatives.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours for Fresh Tissue, Indefinite for FFPE and Unstained slides |