Picrosirius Red Stain
Use
Picrosirius Red (PSR) special stain is used to identify and differentiate collagen fibers, particularly in the context of fibrosis and connective tissue diseases. PSR aids in diagnosing conditions such as liver cirrhosis, myocardial fibrosis, and various connective tissue disorders, providing valuable insights into the degree of fibrosis.
Special Instructions
Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Methodology
Other
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Unknown)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Fresh Tissue tissues: ≤ 72 Hours. Formalin-fixed, Paraffin-Embedded (FFPE) tissues: Indefinite. Unstained slides: Indefinite.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.