Placental alkaline phosphatase, technical only
Use
PLAP is a membrane-associated sialoglycoprotein enzyme normally present within the placenta during the third trimester of gestation and fetal/neonatal testes and thymus. Abnormal expression can be detected in endocervix, fallopian tube, and ovarian tumors as well as germ cell tumors. Technical component only. Pathologist read not included.
Special Instructions
Fresh tissues should be fixed at a 20:1 fixative to tissue ratio in 10% NBF for 6-72 hours (48-72 hours preferred). Formalin-fixed tissues should be stored at 15°C to 25°C. Fresh tissues may be transferred to 70% ethanol and stored at 2-8°C for longer storage. Positively charged glass slides are required for unstained slides to prevent background staining and tissue loss.
Limitations
The test does not include a pathologist read. Only the technical component is provided, limiting the interpretative aspect. Fresh tissues not fixed in 10% NBF or incorrect fixation methods may result in specimen rejection. PLAP testing is not applicable for specimens that do not meet the specified fixation criteria or exceed stability conditions.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 ratio to tissue, using 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C. Specimens can be transferred to 70% ethanol for longer-term storage.
Causes for Rejection
All fixatives other than 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |