PRAME
Use
PRAME is a melanoma-associated antigen that acts as a repressor of the retinoic acid receptor signaling pathway. It is diffusely positive in many melanoma subtypes and other malignant tumors such as certain sarcomas, carcinomas, and hematolymphoid neoplasms. PRAME is basally expressed in benign testis, ovary, placenta, adrenals, and endometrium tissues.
Special Instructions
Fresh tissues must be properly fixed in a 20:1 fixative to tissue ratio using 10% neutral buffered formalin for an appropriate duration. Unstained slides require positively charged glass to avoid background staining and tissue loss, and should remain unbaked.
Limitations
PRAME staining can also occur in tumors other than melanoma, which may affect specificity. Proper tissue fixation and slide preparation are crucial to ensure accurate results.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |