PRAME red, technical only
Use
Preferentially expressed antigen in melanoma (PRAME) is a melanoma-associated antigen that acts as a repressor of the retinoic acid receptor signaling pathway. PRAME stains many melanoma subtypes diffusely positive as well as other malignant tumors such as certain sarcomas, carcinomas, and hematolymphoid neoplasms. PRAME is basally expressed in benign testis, ovary, placenta, adrenals, and endometrium tissues.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Technical component only. PRAME assay utilizes RED chromogen and is limited by the fixation quality of specimens. Specimens must be fixed in 10% NBF or B plus.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |