Prostate specific antigen, technical only
Use
Prostate-specific antigen (PSA) is strongly expressed in both normal and neoplastic prostatic tissues. This antibody is used to determine the prostatic origin of neoplasms in non-prostate tissues, aiding in the identification of metastatic disease. The test is for technical component only and does not include a pathologist read.
Special Instructions
Fresh tissue specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer storage if required. Unstained slides should be positively charged glass slides to prevent background staining and tissue loss. Slides should remain unbaked.
Limitations
The test is limited to detecting the presence of prostate-specific antigen in tissue samples. Optimal fixation conditions must be followed to ensure accurate results. Specimens fixed in solutions other than 10% neutral buffered formalin or B plus will be rejected. Turnaround times can vary based on biopharma protocol requirements and specific study contracts.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |