Prostatic acid phosphatase, technical only
Use
Prostatic acid phosphatase (PSAP) is strongly expressed in both normal and neoplastic prostatic tissues. This antibody is useful in determining prostatic origin of neoplasms in non-prostate tissues (metastatic disease) and differentiating between prostatic and urothelial carcinomas or mesonephric remnant hyperplasia. Technical component only. Pathologist read not included.
Special Instructions
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
The test is limited to the technical component only, and does not include pathologist interpretation. Specimens must be fixed in 10% NBF or B plus, as all other fixatives will be rejected. Specimens must be tested 3 months after placing in ethanol. Collection sites must adhere to prescribed fixation times and storage conditions to ensure sample integrity.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |
| Frozen | Indefinite |