TAG 72
Use
TAG-72 is a glycoprotein expressed on the surface of many epithelial tumor cells, particularly those in gastrointestinal, breast, and ovarian cancers. In pathology, TAG-72 is used to identify carcinomas, especially those of the gastrointestinal tract, and is useful for differentiating these from benign lesions.
Special Instructions
- Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. - Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus; all other fixatives will be rejected. Ambient shipping is recommended, with a refrigerated gel pack to protect from extreme temperatures. Fresh tissues have a stability of ≤ 72 hours, FFPE blocks and unstained slides are stable indefinitely.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Ambiguous recommendations indicate storing at ambient temperatures, possibly transferring to 70% alcohol after fixation for transportation.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |