TdT, technical only
Use
TdT is a DNA polymerase enzyme typically expressed in precursor lymphoid cells involved in hematologic malignancies like acute lymphoblastic leukemia. In pathology, TdT serves to distinguish between types of leukemia and lymphoma, particularly identifying T-cell and B-cell precursor leukemias. This test is technical only and does not include pathologist interpretation.
Special Instructions
For fresh tissues, specimens may be fixed in formalin for 24-72 hours at collection sites before transferring to 70% ethanol for longer storage at 2-8°C, ensuring testing within 3 months. Unstained slides require positively charged glass to prevent background staining and tissue loss, and slides should remain unbaked.
Limitations
The test coverage is technical only, with specific requirements for specimen fixation and storage to ensure accurate testing. Other fixatives than 10% NBF or B plus will lead to sample rejection.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Ambient shipment recommended. After fixation, specimens may be transferred to 70% alcohol for transportation.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |