Thyroid Transcription Factor-1
Use
TTF-1 is a nuclear protein crucial for the development of the thyroid, lung, and brain. TTF-1 is used to differentiate between lung adenocarcinomas and other malignancies, as its expression is strongly positive in non-small cell lung cancer, particularly adenocarcinoma, and in thyroid tumors. It is also valuable in identifying metastatic tumors to the lung, as TTF-1 positivity helps distinguish primary lung cancers from other types of carcinomas.
Special Instructions
For fresh tissues, specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides require positively charged glass slides to prevent background staining and tissue loss.
Limitations
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract. Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |