TM4SF1 ISH
Use
TM4SF1 is a transmembrane protein involved in cell adhesion and signaling, playing a role in cancer cell migration and metastasis. Its overexpression is used to help identify aggressive tumors, particularly in breast cancer, where it correlates with poor prognosis and increased metastatic potential. The assay detects TM4SF1 mRNA using RNAScope ISH technology.
Special Instructions
Specimens should be fixed for 16-36 hours to prevent RNA degradation. Unstained slides require positively charged glass slides to prevent background staining and tissue loss, and should be left unbaked. FFPE blocks or fresh tissue is preferred.
Limitations
For research use only, actual turnaround times may vary based on biopharma protocol requirements, and the final specifications will be determined in the biopharma study contract.
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Container
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-36 hours (24 hours PREFERRED)
Storage Instructions
15°C to 25°C
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 36 Hours |