Trichrome Stain
Use
Trichrome special stain is used to differentiate collagen fibers from other tissue components, typically in tissues affected by fibrosis. This stain is particularly useful in diagnosing conditions such as liver cirrhosis, kidney fibrosis, and other diseases involving excessive collagen deposition.
Special Instructions
Fresh Tissue: Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Samples should be tested 3 months after placing in ethanol. Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Methodology
Other
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Patient Preparation
Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed.
Storage Instructions
15°C to 25°C
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |