Tyrosinase
Use
Tyrosinase is an enzyme involved in the production of melanin, expressed in melanocytes, and serves as a key marker in the diagnosis of melanocytic tumors. In pathology, tyrosinase is used to confirm the diagnosis of melanoma, distinguishing it from other skin cancers and non-melanocytic tumors. Its expression is also utilized to monitor melanoma progression and metastasis.
Special Instructions
Fresh tissues can be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Positively charged glass slides are required to prevent background staining and tissue loss. Slides should remain unbaked.
Limitations
Specimens must be fixed in 10% neutral buffered formalin (NBF) or similar, such as B Plus. All other fixatives are subject to rejection.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B Plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |