Tyrosinase, technical only
Use
Tyrosinase is an enzyme involved in the production of melanin, expressed in melanocytes, and serves as a key marker in the diagnosis of melanocytic tumors. In pathology, tyrosinase is used to confirm the diagnosis of melanoma, distinguishing it from other skin cancers and non-melanocytic tumors. Its expression is also utilized to monitor melanoma progression and metastasis.
Special Instructions
Technical component only. Pathologist read not included. Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. The test requires high-quality specimens to avoid technical failure; inappropriate fixation or handling may lead to invalid results. TAT may subject to change based on biopharma protocol requirements.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed.
Storage Instructions
15°C to 25°C
Causes for Rejection
All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |