Varicella Zoster Virus
Use
Varicella zoster virus (VZV) is a herpesvirus that causes chickenpox (varicella) in primary infections and shingles (zoster) in reactivated infections. It is crucial for diagnosing VZV-related diseases by detecting VZV glycoprotein or immediate-early proteins, confirming active infection or reactivation, especially in skin lesions, ganglia, and tissues affected by shingles or encephalitis.
Special Instructions
Fresh tissues should be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Samples must be tested within 3 months after placing in ethanol. For unstained slides, positively charged glass slides are required to prevent background staining and tissue loss.
Limitations
Test is subject to variability due to biopharma protocol requirements and environmental conditions during shipping. Fixation in non-acceptable reagents may lead to rejection of specimen. Storage and transport conditions could affect test outcomes. Fresh tissue specimens and FFPE blocks have different stability and storage requirements, which can impact the test if not adhered to.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
8 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, using 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
15°C to 25°C
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |
| Refrigerated | Indefinite |